Validation of carbapenemase and extended-spectrum -lactamase multiplex endpoint PCR assays according to ISO 15189
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چکیده
منابع مشابه
Validation of carbapenemase and extended-spectrum β-lactamase multiplex endpoint PCR assays according to ISO 15189.
OBJECTIVES To validate and accredit a set of three multiplex endpoint PCR assays, targeting the most important carbapenemase and minor extended-spectrum β-lactamase (ESBL) resistance genes, according to the international ISO 15189 particular requirements for the quality and competence of medical laboratories. METHODS Specific primers targeting ESBLs and carbapenemases were collected from the ...
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Optimal use of microbiology laboratories is essential to combat the spread of multiply antibiotic-resistant pathogens. This is vital for patient care, as advocated by Jarvis in stressing the importance of active detection and isolation to control methicillin-resistant Staphylococcus aureus and other resistant hospital-acquired pathogens (17). It is also vital for hospital accreditation in the U...
متن کاملValidation of a laboratory and hospital information system in a medical laboratory accredited according to ISO 15189
INTRODUCTION The aim of the study was to present a protocol for laboratory information system (LIS) and hospital information system (HIS) validation at the Institute of Clinical Chemistry and Laboratory Medicine of the Merkur University Hospital, Zagreb, Croatia. MATERIALS AND METHODS Validity of data traceability was checked by entering all test requests for virtual patient into HIS/LIS and ...
متن کاملEmployee engagement vital to ISO 15189 success.
ISO 15189:2007 provides the particular requirements for quality and competence in the medical laboratory. Each clause within the standard provides systems which are built on processes which are, in turn, comprised of tasks. Records and data provide evidence of implementation in term of these requirements. To that end, the standard is sustained by establishment and continual improvement of stand...
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The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 2013
ISSN: 0305-7453,1460-2091
DOI: 10.1093/jac/dkt065